pcdna3  (Addgene inc)

Journal: Tissue & cell

Article Title: Berberine inhibits low shear stress-induced vascular endothelial inflammation via decreasing phosphorylation of Akt and IRF3.

doi: 10.1016/j.tice.2022.101946

Figure Lengend Snippet: Fig. 3. The inhibitory effect of berberine on LSS-induced IRF3 activation and ICAM-1 expression depends on Akt. (A,B). After being transfected with Akt1-WT plasmids, HUVECs were pretreated with or without BBR and then exposed to LSS. ICAM-1 expression and IRF3 phosphorylation were assessed by Western blot (n = 3). (C,D). After being transfected with Akt1-WT plasmids, HUVECs were pretreated with or without BBR and then exposed to LSS. IRF3 location and ICAM-1 expression were analyzed by immunofluorescence (n = 3). Scale bar= 20 µm. Data are presented as mean ± SEM. * p < 0.05 vs. 60 min.

Article Snippet: Akt1 overexpression plasmid (1477 pcDNA3 flag HA Akt1) was Y. Lv et al. Tissue and Cell 79 (2022) 101946 available on the Addgene website.

Techniques: Activation Assay, Expressing, Transfection, Phospho-proteomics, Western Blot, Immunofluorescence

Journal: Tissue & cell

Article Title: Berberine inhibits low shear stress-induced vascular endothelial inflammation via decreasing phosphorylation of Akt and IRF3.

doi: 10.1016/j.tice.2022.101946

Figure Lengend Snippet: Fig. 4. The inhibitory effect of berberine on LSS-induced ICAM-1 expression depends on Akt in C57BL/6 mice. (A). C57BL/6 mice were injected with AAV9-TIE-Akt1 vector or AAV9-TIE-control vector via tail vein. Four weeks later, partially ligated left common carotid arteries and the mice were gavaged with berberine or normal saline for 8 weeks. (B). After the ligation of the left common carotid arteryl, the expression levels of Akt1 and ICAM-1 in intima of the low shear stress region were detected by immunofluorescence assay. (n = 6).Scale bar= 50 µm. Data are presented as mean ± SEM. * p < 0.05 vs. the AAV-ctrl group. #p < 0.05 vs. the AAV- ctrl group.

Article Snippet: Akt1 overexpression plasmid (1477 pcDNA3 flag HA Akt1) was Y. Lv et al. Tissue and Cell 79 (2022) 101946 available on the Addgene website.

Techniques: Expressing, Injection, Plasmid Preparation, Control, Saline, Ligation, Shear, Immunofluorescence